I don’t know how long a Revio takes to run. I suspect most of my readers are not so interested in “seeing my working”. So here are my best guesses on the time taken during each stage of the sequencing process from “sample-to-sequence”: The sample prep times comes from PacBio’s
Thanks for this analyses Nava, getting estimate numbers on the TAT sure is complex!
"It depends on the application" indeed... and that 10h total time (per your flowchart) doesn't include quant, nor sample prep pre-genomic DNA QC (just as an FYI as I'm thinking through this). How much additional time would you estimate these to take? Let's assume say 24 samples for WGS on the Revio (which is about the throughput, they claim 1300 30x WGS / year or 26 per week if a year is 50 weeks).
Perhaps an additional 3h for 24 samples? (I'm assuming something simple for quant like a Qubit, but am not sure how sensitive a Revio or Sequel is to overloading...)
Thanks for this analyses Nava, getting estimate numbers on the TAT sure is complex!
"It depends on the application" indeed... and that 10h total time (per your flowchart) doesn't include quant, nor sample prep pre-genomic DNA QC (just as an FYI as I'm thinking through this). How much additional time would you estimate these to take? Let's assume say 24 samples for WGS on the Revio (which is about the throughput, they claim 1300 30x WGS / year or 26 per week if a year is 50 weeks).
Perhaps an additional 3h for 24 samples? (I'm assuming something simple for quant like a Qubit, but am not sure how sensitive a Revio or Sequel is to overloading...)