London Calling - Protein Sequencing

Some interesting work on protein sequencing was presented by Oxford Nanopore at London Calling.

Oxford have clearly been working on a number of approaches toward sequencing. Here they presented what seems to be the most complete system peptides flanked by DNA handles:

The big news is that they’ve gone from looking at small known sets to more diverse peptide datasets this year. Pushing their training peptide datasets to ~150K sequences.

They then used the amino caller to call a peptides derived from a subset of E.Coli and align them back to the total E.Coli proteome. This appears to have worked well: