PacBio: More Sensor Notes

Yesterday I wrote about the PacBio sensor architecture and discussed some thoughts on the evolution of the Sequel, Sequel IIe and Revio. Today I’m going to discuss the other presentation I mentioned briefly and ponder upon PacBio a little.

First I wanted to mentioned a quick follow up to my subread experiments post. I have some more experiments planned here, but I have tried running DeepConsensus on the CCS/read set with pulse information removed. DeepConsensus essentially failed to produce any improvement in accuracy here, suggesting that¹ most of what DeepConsensus is doing is incorporating information from the pulse durations/time between pulses.

Anyway, back to the chip design. Below and my notes from Mathieu’s presentation in chronological order (of development at PacBio):