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SRN's avatar

Thanks for the writeup Nava.

I'm a bit puzzled how the chemistry will work without ZMWs (if indeed ZMWs are not being used). If you have ~100nm evanescent depth and ~300nmx300nm pixel, that gives a volume of ~0.01um^3 = 10^-20 m^3 = 10^-17L. If you have 1uM nucleotides then you'll have 10^-23 moles or 6 molecules in the volume at a given time, which will contribute quite a bit of noise. You can drop the nucleotide concentration of course but there's a speed cost to that. Unless the goal is to use synchronized reversibly blocked ncuelotides like ILMN wherein the excess can be washed out.

Pretty interesting nonetheless, thanks for surfacing!

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