The NextSeq 550 LEDs And More Dye Experiments

I’ve previously looked at the NextSeq 550 optical system in some detail. It’s impressive in part because it’s built around such low cost components ($30 LEDs, and image sensors). The LEDs appear to come from Luminus, and some documentation suggests they are 521 and 650nm.

Anyway, I figured I should just try things out for myself, as a prelude to playing around a bit more with these modules. Datasheets suggest the following pinout:

It seemed like it should be possible to drive both at ~3V/200mA. So I ringed these up with a lab supply and measured the output using an old spectrometer. I also took measurements at the objective with the emission going through the NextSeq 550 optical system: