Many thanks to those who provided suggestions and assistance after my last post some interesting options appear to be available and all suggestions and comments are much appreciated.
I think we've come full circle now, as the first Solexa 1G's in 2007 had 26 bp readlengths (that is if memory serves correctly). Will never forget my first customer at NHLBI, what an effort it was to keep that instrument running.
Solexa had as part of its 'acceptance criteria' 1 Billion mappable bases (thus the name "1G") but those first instruments were lucky to get 800 Million bases (800 MB) of mappable reads.
Nice recycling. I hope others are inspired by your efforts to bring MiSeqs to labs who wouldn't otherwise have the budget. Of course, the budget to run sequencing is another factor... it comes back to an old adage, "there is no such thing as a free kitten".
I think we've come full circle now, as the first Solexa 1G's in 2007 had 26 bp readlengths (that is if memory serves correctly). Will never forget my first customer at NHLBI, what an effort it was to keep that instrument running.
Solexa had as part of its 'acceptance criteria' 1 Billion mappable bases (thus the name "1G") but those first instruments were lucky to get 800 Million bases (800 MB) of mappable reads.
It is notable that no one ever returned one!
Nice recycling. I hope others are inspired by your efforts to bring MiSeqs to labs who wouldn't otherwise have the budget. Of course, the budget to run sequencing is another factor... it comes back to an old adage, "there is no such thing as a free kitten".