Aug 7, 2022·edited Aug 7, 2022

Hi Nava, this is a very interesting minimalist idea.

Some thoughts: I assume you'll try to keep the nucleotide concentration very low so that simultaneous incorporation of multiple bases is minimized (the first being bleached well before the second is incorporated), as well as the background fluorescence from bulk nucleotides?

Assuming you're capturing the templates on a random n-mer oligo lawn, is there a possibility that more than one n-mer will bind and prime the same template, be simultaneously extended, creating mixed signals?

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