Illumina’s roadmap is now talking about 2x500 reads
But they probably won’t go much further, as 500bp is about the limit for the current patterned flowcells (my wet lab colleagues cued me in to this) - don’t know if that is well geometry or the enzymology, but inserts over that size are disfavored
I agree, but I think as previous demos have shown, they could (and have demonstrated) produce 650bp either on some instruments or on a new instrument which switched up the platform. May have to sacrifice density.
What you described from Roche is 15 years of work, without having to commercialise, it is still materially behind ONT and at best competitive with ILMN or Ultima
It has shorter reads than ONT, doesn’t do direct RNA or modifications.
But I don’t see it as “materially behind” ONT in other respects. Baseline accuracy is better (Q20 average) the raw data is cleaner, and the duplex approach gets you Q39 bases. Of course, there may be other issues that only become apparent after launch.
I would agree Mr Wibble that it is not directly competitive with ONT today as Oxford mostly plays on read length currently.
Mr Wibble. Oxford have been talking about the FET array for many many years. We’ve yet to see any data from this platform.
But this post is mostly about Roche’s, and if the techniques that have been demonstrated there can be applied to a stranded system. I would argue Mr Wibble. That this would be difficult.
The electrochemistry on ONT allows recharging so that on a channel can be reset. Possible on a mux chip to charge on channel and run another or recharge the whole chip.
Illumina’s roadmap is now talking about 2x500 reads
But they probably won’t go much further, as 500bp is about the limit for the current patterned flowcells (my wet lab colleagues cued me in to this) - don’t know if that is well geometry or the enzymology, but inserts over that size are disfavored
I agree, but I think as previous demos have shown, they could (and have demonstrated) produce 650bp either on some instruments or on a new instrument which switched up the platform. May have to sacrifice density.
But I think there is likely limited demand.
What you described from Roche is 15 years of work, without having to commercialise, it is still materially behind ONT and at best competitive with ILMN or Ultima
It has shorter reads than ONT, doesn’t do direct RNA or modifications.
But I don’t see it as “materially behind” ONT in other respects. Baseline accuracy is better (Q20 average) the raw data is cleaner, and the duplex approach gets you Q39 bases. Of course, there may be other issues that only become apparent after launch.
I would agree Mr Wibble that it is not directly competitive with ONT today as Oxford mostly plays on read length currently.
Their FET array has a common trans so no limitation. They show updates on this system every year and it is competitive with the Roche system.
Mr Wibble. Oxford have been talking about the FET array for many many years. We’ve yet to see any data from this platform.
But this post is mostly about Roche’s, and if the techniques that have been demonstrated there can be applied to a stranded system. I would argue Mr Wibble. That this would be difficult.
The electrochemistry on ONT allows recharging so that on a channel can be reset. Possible on a mux chip to charge on channel and run another or recharge the whole chip.
Thank you Mr Wibble. Is this currently implemented?