Late last year I wrote a post claiming that sequencing is more sensitive than qPCR. In that post I looked a few papers I’d recently come across which seemed to show that sequencing is more sensitive than qPCR in those contexts. A reasonable concern is that I looked at a relatively limited number of papers… so, I decided to try and do something
At extremely low concentrations, a perpetual concern in genomics lab is cross-contamination between samples. Hopefully labs that are running lots of qPCR set things up to prevent those sorts of situations, but could the sequence data itself help eliminate cross-contamination? For samples that are negative by qPCR but positive by seq, does the seq data support a lineage different from other samples in the same batch?
I hope to find some time to dive into these papers to see if they have addressed this...
At extremely low concentrations, a perpetual concern in genomics lab is cross-contamination between samples. Hopefully labs that are running lots of qPCR set things up to prevent those sorts of situations, but could the sequence data itself help eliminate cross-contamination? For samples that are negative by qPCR but positive by seq, does the seq data support a lineage different from other samples in the same batch?
I hope to find some time to dive into these papers to see if they have addressed this...