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PacBio's Sensors - Sequel Layouts, Revio /is/ single channel?
Yesterday someone was asking me if PacBio used an off-the-shelf image sensor and built their ZMWs on top of it. My assumption has always been that the…
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Nava Whiteford
2
More PacBio Subread Experiments...
In my last post I took a quick look at some PacBio subreads and used the open source CCS tools to generate the consensus reads. I’ve now run these…
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Nava Whiteford
A Look At Some PacBio Subreads
To review: PacBio uses a process called CCS (cyclic consensus sequencing). Each DNA fragment is read multiple times. This resolves a fundamental issue…
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Nava Whiteford
Some Thoughts On PacBio's Computational Challenges
A few folks have commented on the Discord that the majority of the cost associated with the Revio is due to the compute requirements. This is plausible…
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Nava Whiteford
PacBio News Doesn't Seem /That/ Bad? Does It?
PacBio released preliminary Q1 revenue and then the stock price dropped ~50%. Market cap currently stands at ~$375M: They’ve also said they are going to…
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Nava Whiteford
3
MiSeq Imaging Regions
I wanted to get a rough sense of where the imaging regions on a MiSeq flowcell were. The diagram below shows where the 14 tiles in standard v2 flowcells…
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Nava Whiteford
Why Is Darth Vader On The PacBio Revio Flowcell?
I spent a bit more time looking at heat maps of Revio flowcells after my last post. Taking all the runs from here and averaging over the datasets. This…
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Nava Whiteford
ASeq Newsletter
ASeq Newsletter
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