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PacBio: More Sensor Notes
Yesterday I wrote about the PacBio sensor architecture and discussed some thoughts on the evolution of the Sequel, Sequel IIe and Revio. Today I’m going…
13 hrs ago
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Nava Whiteford
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PacBio: More Sensor Notes
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PacBio's Sensors - Sequel Layouts, Revio /is/ single channel?
Yesterday someone was asking me if PacBio used an off-the-shelf image sensor and built their ZMWs on top of it. My assumption has always been that the…
Apr 25
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Nava Whiteford
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PacBio's Sensors - Sequel Layouts, Revio /is/ single channel?
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More PacBio Subread Experiments...
In my last post I took a quick look at some PacBio subreads and used the open source CCS tools to generate the consensus reads. I’ve now run these…
Apr 23
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Nava Whiteford
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More PacBio Subread Experiments...
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A Look At Some PacBio Subreads
To review: PacBio uses a process called CCS (cyclic consensus sequencing). Each DNA fragment is read multiple times. This resolves a fundamental issue…
Apr 21
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Nava Whiteford
3
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A Look At Some PacBio Subreads
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Some Thoughts On PacBio's Computational Challenges
A few folks have commented on the Discord that the majority of the cost associated with the Revio is due to the compute requirements. This is plausible…
Apr 19
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Nava Whiteford
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Some Thoughts On PacBio's Computational Challenges
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PacBio News Doesn't Seem /That/ Bad? Does It?
PacBio released preliminary Q1 revenue and then the stock price dropped ~50%. Market cap currently stands at ~$375M: They’ve also said they are going to…
Apr 18
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Nava Whiteford
1
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PacBio News Doesn't Seem /That/ Bad? Does It?
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MiSeq Imaging Regions
I wanted to get a rough sense of where the imaging regions on a MiSeq flowcell were. The diagram below shows where the 14 tiles in standard v2 flowcells…
Apr 16
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Nava Whiteford
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MiSeq Imaging Regions
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Why Is Darth Vader On The PacBio Revio Flowcell?
I spent a bit more time looking at heat maps of Revio flowcells after my last post. Taking all the runs from here and averaging over the datasets. This…
Apr 15
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Nava Whiteford
10
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Why Is Darth Vader On The PacBio Revio Flowcell?
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PacBio Heatmaps/Revio Chip Layout
Recently a few people have been asking me about PacBio’s platform. Answers to many of these questions would require raw data. Raw data (“Movies”), or…
Apr 12
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Nava Whiteford
6
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PacBio Heatmaps/Revio Chip Layout
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Oxford Nanopore Patents - Part 3
In my continuing series of Oxford Nanopore’s IP, this time I’m looking at the hairpin IP first filed in 2012 and expiring in 2032. We’ve recently…
Apr 11
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Nava Whiteford
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Oxford Nanopore Patents - Part 3
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About That "Viral RNA-Based Diagnostics: Nanopore System"
I thought I’d take a brief break from Oxford Nanopore patents to look at a recent press release someone posted on the Discord (if you’re not already…
Apr 10
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Nava Whiteford
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About That "Viral RNA-Based Diagnostics: Nanopore System"
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ONT Patents - Part 2
Continuing our review of ONT (Oxford Nanopore Technologies) patents things start to get a bit more interesting. There’s one more “rack mount DNA…
Apr 9
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Nava Whiteford
3
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ONT Patents - Part 2
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